A Modification of the Method for the Measurement of Cerebral Blood Flow Using [14C]Iodoantipyrine in Small Animals
- 1 February 1987
- journal article
- research article
- Published by SAGE Publications in Journal of Cerebral Blood Flow & Metabolism
- Vol. 7 (1) , 124-126
- https://doi.org/10.1038/jcbfm.1987.18
Abstract
We have modified the method for the measurement of cerebral blood flow using [14C]iodoantipyrine in small animals to overcome problems caused by rapid sequential arterial sampling. A second isotope, gamma-emitting [99mTc]pertechnetate, is infused together with [14C]iodoantipyrine. The 99mTc is counted externally by a detector placed over the chest. The arterial curve for [14C]iodoantipyrine can be reconstructed from a single arterial sample collected from the severed neck and the shape of the 99mTc chest curve. Because of the short half-life of 99mTc (6 h), all 14C measurements, including preparation of autoradiograms, may be made 3 days later.Keywords
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