Study of callus growth and organ formation in wheat (Triticum aestivum) tissue cultures

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Abstract
Callus cultures of wheat (Triticum aestivum L.) were established by incubation of segments from root tips, shoots of seedlings, and from rachis with B5 and T media. 2,4,5-Tri-chlorophenoxyacetic acid, Benazolin, and Banvel D (Dicamba) were found to be appropriate growth regulators for initiation and maintenance of wheat callus cultures. Cytokinins inhibited callus growth. This effect was less pronounced with zeatin than with kinetin and benzyladenine. Supplementation of media with cytokinins, however, increased the number of roots formed in the callus. Shoots and complete plants were regenerated from rachis and shoot callus.

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