AN ANTIGEN COMMON TO A WIDE RANGE OF BACTERIA.

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Abstract
In crude water-soluble extracts of P. aeruginosa, 64 antigens can be demonstrated by crossed immunoelectrophoresis in agarose with polyvalent Pseudomonas immunoglobulin [Ig]. One antigen cross-reacts with antigens prepared from bacteria of a wide range of taxonomic groups. Monospecific Ig to this antigen (Common Antigen) were produced by immunization with the appropriate immunocomplex extracted from agarose. Common Antigen was purified by the combination of 2 fractionation methods: precipitation of the crude extract with 18% (wt/vol) sodium sulfate, followed by gel filtration of the supernatant on a Sephadex G-200 column. By this method, 35% of Common Antigen from the crude extract was recovered, more than half of the fractions electrophoretically pure. Electrophoresis of reduced Common Antigen on a sodium dodecyl sulfate polyacrylamide gel revealed 2 protein bands with apparent MW of 59,000-62,000 and 62,000-65,000, respectively. The untreated antigen passed through a column of Sephadex G-200 with the void volume, indicating a substance of high MW (> 400,000-600,000).

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