An Evaluation of Quantitative Precision in High Performance Liquid Chromatography

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Abstract
Results from a cooperative study initiated by ASTM E-19 to evaluate the quality of data to be expected from current practice of liquid chromatography are discussed. Seventy-eight laboratories participated in the program using reversed-phase columns to analyze two samples, each in triplicate. The first sample was an easily separated four-component mixture; the second was a more complex six-component mixture. Mean values of the analytical data submitted were consistent with the known concentrations of the components in each sample, indicating a highly satisfactory degree of overall accuracy. However, the spread of data, expressed as percent relative standard deviation, revealed analytical problems for some laboratories. Relative standard deviations for the whole data set ranged from 6% to 11% in the first sample. The problems were more serious in the more complex sample with relative standard deviations ranging from 9% to 16%. removal of data from outlier laboratories using a multivariate analysis of the data reduced relative standard deviations in the first sample to a range of 3% to 5% and in the second sample to a range of 3% to 8%. This latter information was representative of the performance of about 90% of the participating laboratories.

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