Type‐c virus‐specific nucleic acid sequences in cultured rat cells

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Abstract
Single‐stranded DNA transcripts of rat type‐C viruses prepared in the presence of actinomycin D, hybridized specifically to DNA of several rat cell cultures with no obvious qualitative or quantitative differences. Similar products prepared from a pseudo‐type sarcoma virus with contributions from rat and mouse type‐C viruses hybridized to both rat and mouse cellular DNA, while mouse viral transcripts did not hybridize to rat cell DNA. Viral RNA was detected in all rat cells by means of the rat viral DNA transcripts, with some differences between untreated low‐passage cells and sister cultures treated with bromodeoxyuridine or bromodeoxyuridine and methylchol‐anthrene. Cells treated with both compounds were previously shown to be transformed and tumorigenic, and these were distinguishable by kinetic analysis from the control cells.