Duplication of the MHC-linked Xenopus complement factor B gene
- 1 July 1995
- journal article
- Published by Springer Nature in Immunogenetics
- Vol. 42 (3) , 196-203
- https://doi.org/10.1007/bf00191225
Abstract
We have previously reported the molecular cloning of the mammalian major histocompatibility complex (MHC) class III gene, complement factor B (Bf) from Xenopus laevis, and linkage of the gene to the frog MHC. Here, we estimated the copy number of the Xenopus Bf gene by genomic Southern blotting analysis and demonstrated that Xenopus laevis has two copies of the Bf gene. Both genes co-segregated with the MHC-linked HSP70 genes among 19 offspring of an f/r × f/r cross, indicating a close linkage of the two Bf genes to the frog MHC. Both genes are transcribed and contain open reading frames. When compared with the previously determined cDNA sequence (Xenopus Bf A), the predicted amino acid sequence of the second cDNA species (Xenopus Bf B) shows 82% overall identity. Polymerase chain reaction analysis indicated that all of the partially inbred frogs with the f, r, g, and j MHC haplotypes, as well as 12 outbred frogs tested have both Bf genes, suggesting that the duplicated Bf genes are stable genetic traits in Xenopus laevis.Keywords
This publication has 31 references indexed in Scilit:
- Human complement factor B: cDNA cloning, nucleotide sequencing, phenotypic conversion by site-directed mutagenesis and expressionMolecular Immunology, 1993
- Isolation of a classical MHC class I cDNA from an amphibian. Evidence for only one class I locus in the Xenopus MHC.The Journal of Immunology, 1993
- Evolution of duplicate genes in a tetraploid animal, Xenopus laevis.Molecular Biology and Evolution, 1993
- Evidence that gene G7a in the human major histocompatibility complex encodes valyl-tRNA synthetaseBiochemical Journal, 1991
- Murine complement component C4 and sex-limited protein: identification of amino acid residues essential for C4 function.Proceedings of the National Academy of Sciences, 1989
- Molecular analysis of human complement component C5: localization of the structural gene to chromosome 9Biochemistry, 1988
- Common evolutionary origin of alpha 2-macroglobulin and complement components C3 and C4.Proceedings of the National Academy of Sciences, 1985
- Mapping of steroid 21-hydroxylase genes adjacent to complement component C4 genes in HLA, the major histocompatibility complex in man.Proceedings of the National Academy of Sciences, 1985
- Purification of a lamprey complement protein homologous to the third component of the mammalian complement system.The Journal of Immunology, 1984
- TheC4andSlpgenes of the complement region of the murineH-2major histocompatibility complexPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1984