Plaque Formation by Arboviruses
Open Access
- 1 March 1968
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 2 (2) , 273-284
- https://doi.org/10.1099/0022-1317-2-2-273
Abstract
Cell lines of baby hamster kidney (BHK 21), green monkey kidney (Vero, MA 134), rhesus monkey kidney (MA 104), and rabbit kidney (MA 111) were found suitable for titration and multiplication of arboviruses. Plaque characteristics of 52 arboviruses and 20 virus strains and titres of some of them in BHK 21 and other cell lines were established, using one type of serum-free standard overlay and standard maintenance medium. However, the addition of cortisol or other compounds was necessary to prolong survival of BHK 21 cell sheets, and induce or improve plaque formation.Keywords
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