Stabilisation of a yeast artificial chromosome containing plant DNA using a recombination-deficient host
- 1 March 1993
- journal article
- update section
- Published by Springer Nature in Plant Molecular Biology
- Vol. 21 (6) , 1187-1189
- https://doi.org/10.1007/bf00023615
Abstract
The large genomes of many plant species contain numerous dispersed repeat sequences. The problem of large-scale structural instability due to the presence of such repeats in yeast artificial chromosome (YAC) clones was assessed. The feasibility of stabilising plant sequences prone to rearrangement in YACs was demonstrated using a host yeast strain deficient in recombination.Keywords
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