RAPID PRODUCTION OF COMPLEMENT FIXATION ANTIGENS FOR SYSTEMIC MYCOTIC DISEASES I
Open Access
- 1 June 1959
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 77 (6) , 753-756
- https://doi.org/10.1128/jb.77.6.753-756.1959
Abstract
Five different media were tested for their ability to produce complement-fixation antigens from Coccidioides immitis. The cultures were incubated while stationary at 25[degree]C or were continuously agitated at 25[degree]C for 1 to 4 weeks. A neopeptone dialyzate medium with agitation produced the most satisfactory antigen, after 3 weeks of growth. It was as antigenic as the standard coccidioidin, free of anticomplementary activity, and was readily reproducible. This complement-fixation antigen lacked skin test reactivity.Keywords
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