Structural basis for the guanine nucleotide-binding activity of tissue transglutaminase and its regulation of transamidation activity
Top Cited Papers
Open Access
- 26 February 2002
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 99 (5) , 2743-2747
- https://doi.org/10.1073/pnas.042454899
Abstract
Tissue transglutaminase (TG) is a Ca2+-dependent acyltransferase with roles in cellular differentiation, apoptosis, and other biological functions. In addition to being a transamidase, TG undergoes a GTP-binding/GTPase cycle even though it lacks any obvious sequence similarity with canonical GTP-binding (G) proteins. Guanine nucleotide binding and Ca2+ concentration reciprocally regulate TG's transamidation activity, with nucleotide binding being the negative regulator. Here we report the x-ray structure determined to 2.8-Å resolution of human TG complexed with GDP. Although the transamidation active site is similar to those of other known transglutaminases, the guanine nucleotide-binding site of TG differs markedly from other G proteins. The structure suggests a structural basis for the negative regulation of transamidation activity by bound nucleotide, and the positive regulation of transamidation by Ca2+.Keywords
This publication has 47 references indexed in Scilit:
- GTP Binding and Signaling by Gh/Transglutaminase II Involves Distinct Residues in a Unique GTP-binding PocketPublished by Elsevier ,2000
- The Ras-RasGAP Complex: Structural Basis for GTPase Activation and Its Loss in Oncogenic Ras MutantsScience, 1997
- [20] Processing of X-ray diffraction data collected in oscillation modePublished by Elsevier ,1997
- C-terminal Deletion of Human Tissue Transglutaminase Enhances Magnesium-dependent GTP/ATPase ActivityJournal of Biological Chemistry, 1996
- Biochemical Effects of Retinoic Acid on GTP-binding Protein/Transglutaminases in HeLa CellsJournal of Biological Chemistry, 1996
- Tissue Transglutaminase and Factor XIII in Cartilage and Bone RemodelingSeminars in Thrombosis and Hemostasis, 1996
- The importance of the GTP‐binding protein tissue transglutaminase in the regulation of cell cycle progressionFEBS Letters, 1995
- The structure of transducin Gαt: More to view than just RasCell, 1994
- Localization of cellular transglutaminase on the extracellular matrix after wounding: Characteristics of the matrix bound enzymeJournal of Cellular Physiology, 1991
- Regulation of tissue transglutaminase gene expression as a molecular model for retinoid effects on proliferation and differentiationJournal of Cellular Biochemistry, 1989