Effect of temperature on virus eradication and growth of infected tissue cultures

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Abstract
SUMMARY: Cucumber mosaic (CMV) and alfalfa mosaic (AlfMV) viruses were eradicated or their concentrations greatly diminished when infected meristem tips were grown in static or shake culture at 32 or 34 oC. Similar cultures grown at 22 oC remained infected. Tobacco mosaic virus (TMV) was not eradicated from shake cultures at 32 oC and the concentration of TMV in such cultures at 32 oC was sometimes higher than at 22 oC.The concentration of CMV and AlfMV was considerably less in cultured tissues than in infected leaf material, but that of TMV was similar. Cultures infected with CMV or TMV grew more slowly than healthy cultures. Growth of AlfMV infected and healthy cultures was similar.