THE ISOLATION OF HISTOPLASMA CAPSULATUM FROM TISSUES OF EXPERIMENTALLY INFECTED MICE

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Abstract
The efficiency of two basic technics for the demonstration of H. capsulatum in infected mice is evaluated. The technics evaluated are Leishman stained tissue impressions of liver, spleen, and heart blood, and the culture of these same tissues on 5 different media. H. capsulatum was cultured from every mouse by the simultaneous use of 5 different media. H. capsulatum was observed in stained tissue impressions of liver, spleen, and heart blood in 85.7% of the mice. The fungus was observed with equal frequency (78.5%) in the liver and spleen. Heart blood, however, revealed the fungus in only 11.9% of the animals. The efficiency of the 5 media used for the cultural isolation is in the following order with the most efficient one first: brain-heart infusion blood agar, Sabouraud''s dextrose agar, brain-heart infusion agar, potato-dextrose agar, and mycophil agar. The highest percentage of cultural isolations was obtained with spleen inoculation on Sabouraud''s dextrose agar. A high degree of simultaneously infected livers and spleens from the same animals was shown to exist by microscopic and cultural demonstration of the fungus.
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