Carbon monoxide- and oxygen-reacting haemoproteins in the mitochondrial fraction from the soil amoeba Acanthamoeba castellanii. Studies at subzero temperatures

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Abstract
Mitochondria-enriched fractions of the soil amoeba A. castellanii contained 4 hemoproteins that in their reduced forms reacted with CO to give photodissociable CO complexes; these were cytochromes a3 and a614 as well as b- type and c-type cytochromes. Non-photodissociable oxygen-containing compounds were formed at temperatures between -130 and -150.degree. C after photodissociation of CO in the presence of 200 .mu.M O2. Electron transport, indicated by the oxidation of cytochromes a + a3 and cytochrome c, did not occur until the temperature was raised to -80.degree. C.