Measurement of the Opsonic Effect of Normal and Immune Sera on the Phagocytosis of Salmonella Typhi by the Reticuloendothelial System
Open Access
- 1 September 1961
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 87 (3) , 296-300
- https://doi.org/10.4049/jimmunol.87.3.296
Abstract
Summary: A precise and sensitive method is described for measuring the opsonic activity of a serum on the phagocytosis of Salmonella typhi by the reticuloendothelial system in mice. This method is based upon the measurement of the increased rate of phagocytosis of circulating microorganisms, produced by the injected opsonins. The serum activity is expressed in terms of opsonizing units/ml (OpU/ml). The opsonin titer of eight normal rabbit sera ranged between 11 and 250 OpU/ml. The immunization of rabbits with H antigen did not significantly increase the opsonizing activity of their serum as evidenced by an opsonic titer ranging from 31 to 215 OpU/ml. The immunization with O antigen markedly increased the opsonic titer of the serum to 18,000 to 48,000 OpU/ml.Keywords
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