The antibody response of pigs to dinitrophenylated bovine IgG administered with a novel type of adjuvant

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Abstract
High and reproducible antibody response to miligram quantities of 2,4‐dinitrophenyl (DNP)‐conjugated bovine IgG was elicited in pigs with the aid of a novel adjuvant composed of aluminium hydroxide, paraffin oil and Sorbitan trioleate (Al‐Span‐Oil). Repeated injections of antigen produced antibody levels up to 8 mg per ml of serum. The antibody level reached the maximum around the 12th day after first antigen injection, but around the 7th day after subsequent antigen injection. Isolated anti‐DNP antibody was found to be chemically heterogeneous. It bound ϵ‐DNP‐lysine with a mean association constant of about 106 M−1.