Partial Amino‐Acid Sequence of the Epidermal Growth‐Factor‐Binding Protein

Publisher Website
Google Scholar
Abstract
The partial amino acid sequence of the epidermal growth‐factor‐binding protein was determined. Residues in 108 unique positions, corresponding to 45% of the molecule, were identified. The protein is a serine protease, closely related to the nerve growth factor γ subunit. It is suggested that the epidermal growth‐factor‐binding protein, like other serine protease, is synthesized as a single polypeptide chain which undergoes limited endoproteolysis. The isolated material also contained minor amounts of a second serine protease. This protease is closely related to the epidermal growth‐factor‐binding protein, differing from it in 7 out of the 45 amino acid positions available for comparison. The latter protease may be identical to the previously described protease A.