Cloning and expression of a new cDNA from monocotyledonous plants coding for a diadenosine 5′,5′′′‐P1,P4‐tetraphosphate hydrolase from barley (Hordeum vulgare)
- 24 July 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 431 (3) , 481-485
- https://doi.org/10.1016/s0014-5793(98)00819-9
Abstract
From a cDNA library generated from mRNA of white leaf tissues of the ribosome-deficient mutant ‘albostrians' of barley (Hordeum vulgare cv. Haisa) a cDNA was isolated carrying 54.2% identity to a recently published cDNA which codes for the diadenosine-5′,5′′′-P1,P4-tetraphosphate (Ap4A) hydrolase of Lupinus angustifolius (Maksel et al. (1998) Biochem. J. 329, 313–319), and 69% identity to four partial peptide sequences of Ap4A hydrolase of tomato. Overexpression in Escherichia coli revealed a protein of about 19 kDa, which exhibited Ap4A hydrolase activity and cross-reactivity with an antibody raised against a purified tomato Ap4A hydrolase (Feussner et al. (1996) Z. Naturforsch. 51c, 477–486). Expression studies showed an mRNA accumulation in all organs of a barley seedling. Possible functions of Ap4A hydrolase in plants will be discussed.Keywords
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