THE EFFECT OF PROSTAGLANDIN-I2, PROSTAGLANDIN-E1, PROSTAGLANDIN-E2 AND DIBUTYRL CYCLIC-AMP ON THE CYTOPLASMIC SPREADING OF RAT OSTEOCLASTS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 65  (5) , 557-566
Abstract
Osteoclasts were isolated from neonatal rat bone and assessed the effects of prostaglandins (PG) I2, E1 and E2 on osteoclastic spreading. Although the PG and dibutyryl cAMP (DB-cAMP) acted as direct inhibitors of osteoclastic spreading, if osteoblasts and osteoclasts were co-cultured, the addition of PG or DB-cAMP caused a considerable increase in spreading. The PG and DB-cAMP apparently induce osteoblasts to stimulate osteoclasts. Osteoblasts are known to produce PG, and possess the capacity to either inhibit (through PG) or stimulate osteoclasts. The balance between stimulation and inhibition of osteoclasts by osteoblasts may be determined by osteoblastic cAMP levels.