The Storage of Fertilized Sheep Ova At 5°C
Open Access
- 1 January 1973
- journal article
- Published by CSIRO Publishing in Australian Journal of Biological Sciences
- Vol. 26 (6) , 1421-1428
- https://doi.org/10.1071/bi9731421
Abstract
Fertilized day-2 (1-4 cells) and day-5 (morulae) sheep ova were cooled at a rate of 0�25 or 1�0 degC/min to 5�C then stored at 5�C for intervals between 10 min and 6 hr, after which they were cultured in vitro for two days at 37� 5�C. Storage had no effect upon subsequent development in culture. Of 103 day-2 ova, 75 (73 %) developed to eight cells and 57 of 88 (65 %) day-5 ova developed to blastocysts. There were no effects of age of ovum, cooling rate, or duration of storage on development in culture.Keywords
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