Diverse Tetracycline Resistance Genotypes of Megasphaera elsdenii Strains Selectively Cultured from Swine Feces
- 1 June 2004
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 70 (6) , 3754-7
- https://doi.org/10.1128/aem.70.6.3754-3757.2004
Abstract
A total of 30 Megasphaera elsdenii strains, selectively isolated from the feces of organically raised swine by using Me109 M medium, and one bovine strain were analyzed for tetracycline resistance genotypic and phenotypic traits. Tetracycline-resistant strains carried tet (O), tet (W), or a tet gene mosaic of tet (O) and tet (W). M. elsdenii strains carrying tet (OWO) genes exhibited the highest tetracycline MICs (128 to >256 μg/ml), suggesting that tet (O)- tet (W) mosaic genes provide the selective advantage of greater tetracycline resistance for this species. Seven tet genotypes are now known for M. elsdenii , an archetype commensal anaerobe and model for tet gene evolution in the mammalian intestinal tract.Keywords
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