A Water-Soluble Antitumor Glycoprotein fromChlorella vulgaris
- 1 October 1996
- journal article
- research article
- Published by Georg Thieme Verlag KG in Planta Medica
- Vol. 62 (05) , 423-426
- https://doi.org/10.1055/s-2006-957931
Abstract
An active substance with antitumor activity (ARS2) was purified from the culture media of Chlorella vulgaris and found to be a glycoprotein with a molecular weight of 63,100 amu, as determined by matrix-assisted laser desorption/ ionization (MALDI) mass spectrometry. ARS2 contains 66.9% carbohydrate, mainly D-galactose, and 35.2% protein. The carbohydrate moiety has a β-1,6-D-galactopyranose backbone, as determined by methylation analysis and 13C-NMR. Apparently, the protein moiety, whose 15 amino acid sequence at the NH2-terminus, we determined as DVGEAFPTWDALVA, is necessary for the antitumor activity, as assessed by hydrazinolysis, periodate oxidation, and proteolysis.Keywords
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