Distinct effects of acetylcholine and glucose on 45calcium and 86rubidium efflux from mouse pancreatic islets
- 29 October 1984
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 176 (2) , 457-461
- https://doi.org/10.1016/0014-5793(84)81218-1
Abstract
The similarities between the effects of acetylcholine and glucose on phospholipid metabolism in pancreatic islet cells prompted the comparison of their effects on ionic fluxes. Acetylcholine (1 μM) consistently increased 45Ca2+ efflux from mouse islets, whereas glucose increased it in the presence, but decreased it in the absence of extracellular Ca2+. Acetylcholine consistently accelerated 86Rb+ efflux, and this effect was augmented by Ca2+ omission. On the other hand, glucose markedly inhibited 86Rb+ efflux, except when its concentration was raised from 10 to 15 mM in the presence of Ca2+. Unlike their effects on phospholipid metabolism, the ionic effects of the two insulin-secretagogues are thus very different.Keywords
This publication has 21 references indexed in Scilit:
- Rapid mobilization of Ca2+ from rat insulinoma microsomes by inositol-1,4,5-trisphosphateNature, 1984
- Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic β‐cellsFEBS Letters, 1984
- Stimulation of phosphoinositide breakdown in rat pancreatic islets by glucose and carbamylcholineBiochemical and Biophysical Research Communications, 1983
- Role of Ca2+ in secretagogue-stimulated breakdown of phosphatidylinositol in rat pancreatic islets.Journal of Clinical Investigation, 1983
- Phosphatidylinositol and phosphatidic acid metabolism in rat pancreatic islets in response to neurotransmitter and hormonal stimuliBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
- Stimulus-excitation coupling in plasma membranes of pancreatic acinar cellsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1982
- Opposite effects of intracellular Ca2+ and glucose on K+ permeability of pancreatic islet cellsNature, 1979
- D-Glucose inhibits potassium efflux from pancreatic islet cellsNature, 1978
- Effects of insulin secretagogues on phospholipid metabolism in pancreatic β-cellsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1975
- Inositol phospholipids and cell surface receptor functionBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1975