Creation of a data base for sequences of ribdsomal nucleic acids and detection of conserved restriction endonucleases sites through computerized processing

Open Access
Publisher Website
Google Scholar
Abstract
As part of a project pertaining the organization of riboscmal genes in Kinetoplastidae, we have created a data base for published sequences of ribosomal nucleic acids, with information in Spanish. As a first step in their processing, we have written a computer program which introduces the new feature of determining the length of the fragments produced after single or multiple digestion with any of the known restriction enzymes. With this information we have detected conserved SAU 3A sites: (i) at the 5′ end of the 5.8S rRNA and at the 3′ end of the small subunit rRNA, both included in similar larger sequences; (ii) in the 5.8S rRNA of vertebrates (a second one), which is not present in lower eukaryotes, showing a clear evolutive divergence; and, (iii) at the 5′ terminal of the small subunit rRNA, included in a larger conserved sequences. The possible biological importance of these sequences is discussed.