Abstract
Carbon magnetic resonance establishes conclusively that 6 polyene macrolide antibiotics containing keto groups (the heptaene amphotericin B, the tetraene-diene nystatin A1 and the tetraenes tetrin A, tetrin B, pimaricin and lucensomycin) exist in the hemiketal form in solution. Their spectra all contain a hemiketal C''s absorption near 97 ppm but lack a keto C''s absorption near 210 ppm. The non-polyenic macrolide erythromycin exists in the keto form.

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