Improvements in the Growth of BHK-21 Cells in Submerged Culture
Open Access
- 1 January 1971
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 22 (4) , 534-537
- https://doi.org/10.1128/aem.22.4.534-537.1971
Abstract
A medium is described which will support the submerged culture of BHK-21 cells to 6.5 × 106 to 7 × 106 cells/ml in volumes up to 30 liters. Shaken-flask cultures were used to determine nutrient deficiencies in depleted medium. The final medium became limited by glutamine at 6.5 × 106 to 7 × 106 cells/ml, but increasing the glutamine concentration failed to improve cell yields. The value of the polyol Pluronic F68 as a protective substance is illustrated.Keywords
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