Mechanismen der Proteinsynthese, II. Reinigung der Ribosomen ausEscherichia colivon Nucleasen und deren Charakterisierung
- 1 January 1968
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 349 (1) , 54-64
- https://doi.org/10.1515/bchm2.1968.349.1.54
Abstract
After purification of E. coli A 19 ribosomes by 5-7 centrifugations with 0. 5 M NH4CI in standard buffer and subsequent dialysis against 0. 2mM MnCl2, the remaining ribonucleolytic activity is reduced to 10-3-10-4 times its original level. The nucleolytic activity washed away corresponds to RNase II. A residual, apparently exo-nucleolytic activity, remaining on the ribosomes after 5 times washing with NH4O differs in its ion requirement from nucleolytic enzymes previously known.This publication has 17 references indexed in Scilit:
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