AarI, a restriction endonuclease from Arthrobacter aurescens SS2-322, which recognizes the novel non-palindromic sequence 5'-CACCTGC(N)4/8-3'
Open Access
- 1 November 2002
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 30 (21) , 123e-123
- https://doi.org/10.1093/nar/gnf122
Abstract
A new type II restriction endonuclease AarI has been isolated from Arthrobacter aurescens SS2‐322. AarI recognizes the non‐palindromic heptanucleotide sequence 5′‐CACCTGC(N)4/8‐3′ and makes a staggered cut at the fourth and eighth bases downstream of the target duplex producing a four base 5′‐protruding end. AarI activity is stimulated by oligodeoxyribonucleotide duplexes containing an enzyme‐specific recognition sequence.Keywords
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