Depolarizing Agents Regulate the Phosphorylation of Myelin Basic Protein in Rat Optic Nerves

Publisher Website
Google Scholar
Abstract
The regulation of the state of phosphorylation of myelin basic protein was studied in intact rat optic nerves incubated in vitro. The endogenous state of phosphorylation was preserved and the back-phosphorylation technique was used to determine the amount of dephosphorylated protein present in extracts of the nerves. Results indicate that when nerves were incubated in the presence of depolarizing agents, the state of phosphorylation of myelin basic protein was increased. This effect was Ca-dependent and was partly inhibited by chlorpromazine.