Inhibition of Embryonic Cell Aggregation by Neoplastic Cells2
- 1 May 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 54 (5) , 1097-1102
- https://doi.org/10.1093/jnci/54.5.1097
Abstract
The effects of normal and malignant cells on the aggregation of embryonic cells in gyratory shaker cultures were compared. The addition of 1×105 simian virus 40 (SV40)-transformed BALB/3T3 (SV40-3T3) cells to 6X106 embryonic neural retina cells caused a highly significant greater reduct on (22.7%) in aggregate diameter than the addition of untransformed BALB/3T3 (3T3) cells. The ratio of the number of single cells to the number of aggregates was significantly higher for cultures containing SV40-3T3 cells than for the cultures containing 3T3 cells. This effect was concentration dependent in the presence of cultured Ehrlich-Lettré hyperdiploid (ELD) ascites cells; however, media from ELD cell cultures or ELD cell sonicates resulted in aggregates of greater diameter and lower ratios of single cells to aggregates. This approach may provide a sensitive assay system for the interactions of tumor and other cells in vitro.Keywords
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