A MONOCLONAL-ANTIBODY TO A HUMAN-BREAST TUMOR PROTEIN RELEASED IN RESPONSE TO ESTROGEN

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (6) , 2704-2710
Abstract
A panel of monoclonal antibodies was produced against cell surface antigens of the MCF-7 human breast cancer cell line. The monoclonal antibodies were selected by their ability to bind to live, intact MCF-7 cells in solid-phase radioimmunoassay, and to bind to human breast cancer cells in paraffin sections. One monoclonal antibody, designated UCD/AB 6.11, identified 2 cellular antigens and 1 extracellular antigen from MCF-7, and bound to 18 of 20 breast cancers in paraffin sections. The 2 cellular antigens were associated with MW 54,000 and MW 56,000 proteins, which could be identified in Western blots, and were localized to the cell surface by immune precipitation of lactoperoxidase-iodinated plasma membrane proteins. The extracellular antigen, a MW 52,000 protein, was slightly more acidic and was only found in the media from estrogen-stimulated cells. Estrogen did not appear to have an effect on the production of the cell-associated antigens, MW 54,000 and 56,000 proteins. The cellular antigens could be precursors to the secreted MW 52,000 protein.

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