SPECIFIC ABSORPTION OF LYMPHOCYTES-T COMMITTED TO SOLUBLE-PROTEIN ANTIGENS BY INCUBATION ON ANTIGEN-PULSED MACROPHAGE MONOLAYERS

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 123  (4) , 1755-1762
Abstract
Monolayers of macrophages (M.vphi.) pulsed with antigen were used as immunosorbents for T [thymus-derived] lymphocytes from guinea pigs primed to soluble protein antigens. T lymphocytes were cultured on the M.vphi. monolayers for 4 h, then aspirated and reincubated on a fresh monolayer pulsed with the same antigen for a 2nd and a 3rd step. T lymphocytes so treated were selectively deprived of cells responding in assay for antigen-dependent proliferation against the antigen used for pulsing the absorbing monolayer, but maintained their response to other antigens. The lymphocytes adhering to the M.vphi. of the absorbing monolayer were capable of giving a full response to the antigen used for pulsing the M.vphi. of the monolayers. The proliferative response of F1 T lymphocytes to antigen in association with M.vphi. of either parental strain could be absorbed leaving the response to antigen in association with M.vphi. of the other parental strain. The absorption of the proliferative response was not inhibited by addition of excess soluble antigen to the medium of the absorption culture. The results indicate that specific guinea pig T lymphocytes responding by proliferation to soluble protein antigens recognize and bind specifically to a complex of Ia [immune-response-associated] antigen and protein antigen at the surface of the M.vphi.

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