Peroxidase activity in human granulocytes, inter- and intra-individual variations
- 1 January 1977
- journal article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 37 (2) , 163-167
- https://doi.org/10.1080/00365517709156072
Abstract
A kinetic method for quantitation of peroxidase activity (‘myeloperoxidase’, E.C.1.11.1.7.) in human granulocytes has been evaluated. The enzymic activity was determined at 37d`C, pH = 7.0. Substrates: 2-methyxo-phenol (1.25 mmol/l) and H2O2 (0.1 mmol/1). When applied on granulocytes from solitary blood samples from healthy, normal subjects the detected peroxidase activity varied considerably from one person to another (CV = 40–50%). Sequential determinations of the peroxidase activity in granulocytes from selected healthy, normal subjects demonstrated substantial intra-individual variations. Analysis of variance confirmed that the intra-individual variations (CV = 36%) exceeded all other variance components involved.Keywords
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