Negative-strand RNA viruses: genetic engineering and applications.
- 15 October 1996
- journal article
- review article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 93 (21) , 11354-11358
- https://doi.org/10.1073/pnas.93.21.11354
Abstract
The negative-strand RNA viruses are a broad group of animal viruses that comprise several important human pathogens, including influenza, measles, mumps, rabies, respiratory syncytial, Ebola, and hantaviruses. The development of new strategies to genetically manipulate the genomes of negative-strand RNA viruses has provided us with new tools to study the structure-function relationships of the viral components and their contributions to the pathogenicity of these viruses. It is also now possible to envision rational approaches--based on genetic engineering techniques--to design live attenuated vaccines against some of these viral agents. In addition, the use of different negative-strand RNA viruses as vectors to efficiently express foreign polypeptides has also become feasible, and these novel vectors have potential applications in disease prevention as well as in gene therapy.Keywords
This publication has 28 references indexed in Scilit:
- A Cosmid-Based System for Constructing Mutants of Herpes Simplex Virus Type 1Virology, 1993
- Rescue of Synthetic Analogs of Genome RNA of Human Parainfluenza Virus Type 3Virology, 1993
- Alterations of the stalk of the influenza virus neuraminidase: deletions and insertionsVirus Research, 1993
- Priming with recombinant influenza virus followed by administration of recombinant vaccinia virus induces CD8+ T-cell-mediated protective immunity against malaria.Proceedings of the National Academy of Sciences, 1993
- The Basic Science of Gene TherapyScience, 1993
- Rescue of synthetic analogs of respiratory syncytial virus genomic RNA and effect of truncations and mutations on the expression of a foreign reporter gene.Proceedings of the National Academy of Sciences, 1991
- Rescue of a foreign gene by Sendai virus.Proceedings of the National Academy of Sciences, 1991
- Introduction of site-specific mutations into the genome of influenza virus.Proceedings of the National Academy of Sciences, 1990
- Epstein-Barr virus nuclear protein 2 is a key determinant of lymphocyte transformation.Proceedings of the National Academy of Sciences, 1989
- Vaccinia virus: a selectable eukaryotic cloning and expression vector.Proceedings of the National Academy of Sciences, 1982