Site‐directed mutagenesis of ricin A chain Trp 211 to Phe
- 1 April 1990
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 35 (4) , 365-366
- https://doi.org/10.1111/j.1399-3011.1990.tb00062.x
Abstract
Oligonucleotide‐directed site‐specific mutagenesis has been used to make a conservative change in the putative active site of the catalytic subunit of the toxin ricin. The substitution of phenylalanine for tryptophan at position 211 of the A chain reduces but does not abolish catalytic activity, as assayed by inhibition of cell‐free protein synthesis.Keywords
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