Intermediate states between P* and Pf in bacterial reaction centers, as detected by the fluorescence kinetics

Abstract
The fluorescence lifetimes of the reaction centers isolated from the wild‐typeRhodopseudomonas sphaeroidespurple bacterium and those from the R26 mutant strain, lacking the carotenoid, were measured at low redox potential. In addition to the prompt fluorescence occurring directly from P* and the long delayed emission related to the radical pair state Pf, two other components are present. We suggest that they may come from intermediate states between P* and Pf, or reflect the stabilization of Pf itself.

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