MEASUREMENT OF ENDOTOXIN .1. FUNDAMENTAL STUDIES ON RADIOIMMUNOASSAY OF ENDOTOXIN

  • 1 January 1976
    • journal article
    • research article
    • Vol. 30  (4) , 245-255
Abstract
Improvements in the speed and sensitivity of an endotoxin measurement method are described. Antigen was purified from Escherichia coli O111: B4 (B) lipopolysaccharide by centrifugation and dialysis. Purified anti-endotoxin antibody was prepared from immunized rabbit serum. A radioimmunoassay system was established with the antigen and antibody. Dextran-coated charcoal was used to separate the antibody-bound antigen from free antigen. Experimental studies were also performed on possible factors related to the antigen-antibody reaction. Accurate measurements of quantities as low as 100 pg/ml (10 ng/ml in the plasma) were performed by the dextran-coated charcoal method, and the reaction time was reduced to 2 h at 4.degree. C. This new method does not require strict sterilization or aseptic handling and is quite practical for quantitative measurements of endotoxin.

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