A Rapid, Simplified Method for Serial Blood Volume Determinations in the Rat.

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Abstract
Using Evans blue dye and the Beckman spectrophotometer, the method descr. by Cartland and Koch was modified to permit relatively rapid, simplified blood vol. detns. in the rat. Using cardiac puncture, a standard sample was withdrawn and the dye was introduced directly into the left ventricle. The needle was left in place for a 2-min. period before the unknown sample was obtained. The hematocrits were detd. Equal amts. of the dye-free and unknown plasma were diluted, using solns. of Evans blue dye in [image] saline and [image] saline, respectively. Using the spectrophotometer, the density of each sample was detd. With these readings, the dilution factor and the animal wt., the plasma vol., the total blood vol., and the blood vol./100 g. were readily obtained. The blood vol. ranged between 4. and 6.43 cc/100 g. of body wt. These findings compared favorably with the results obtained by other methods. The mortality rate for the first 148 detns. was 4.05%. The usual sources of error native to this type of procedure were controlled by allowing a sufficient dye mixing time, the use of max. spectral absorption range for the dye (620 m[mu]), and diet.

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