Multiphoton Autofluorescence and Second-Harmonic Generation Imaging of the Ex Vivo Porcine Eye

Abstract
Purpose. The purpose of this work was to demonstrate the use of the combined imaging modality of multiphoton autofluorescence and second-harmonic generation (SHG) microscopy in obtaining spectrally resolved morphologic features of the cornea, limbus, conjunctiva, and sclera in whole, ex vivo porcine eyes.

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