MOLECULAR CHARACTERIZATION OF RO(SSA) AND LA(SSB) PROTEINS
- 1 April 1986
- journal article
- research article
- Vol. 13 (2) , 327-330
Abstract
Ro(SSA) and La(SSB) proteins were characterized using human spleen extract as antigen and monospecific anti-Ro(SSA) and anti-La(SSB) sera. The antigens were partially purified by DEAE cellulose and Sephacryl chromatography, and then were electrotransferred to nitrocellulose sheets after SDS-PAGE electrophoresis. The anti-Ro(SSA) sera had reacted with a polypeptidic band of 58 kilodaltons (kDa), meanwhile the La(SSB) antisera reacted with 2 polypeptidic bands of 42 and 40 kDa. The molecular weight of the Ro(SSA) and La(SSB) proteins obtained by gel filtration chromatography was in agreement with the PAGE/electrotransfer results. These procedures are useful tools for the molecular analysis of the markers in the connective tissue diseases.This publication has 3 references indexed in Scilit:
- Partial immunochemical characterization of the Ro and La proteins using antibodies from patients with the sicca syndrome and lupus erythematosus.The Journal of Immunology, 1984
- A host protein (La) binds to a unique species of minus-sense leader RNA during replication of vesicular stomatitis virus.Proceedings of the National Academy of Sciences, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979