Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis
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Open Access
- 1 January 2013
- journal article
- Published by Wiley in Journal of Extracellular Vesicles
- Vol. 2 (1)
- https://doi.org/10.3402/jev.v2i0.19671
Abstract
Nanoparticle tracking analysis (NTA) is a light‐scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.Keywords
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