Role of the Guanosine Triphosphatase Rac2 in T Helper 1 Cell Differentiation
- 23 June 2000
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 288 (5474) , 2219-2222
- https://doi.org/10.1126/science.288.5474.2219
Abstract
T helper 1 (TH1) cells mediate cellular immunity, whereas TH2 cells potentiate antiparasite and humoral immunity. We used a complementary DNA subtraction method, representational display analysis, to show that the small guanosine triphosphatase Rac2 is expressed selectively in murine TH1 cells. Rac induces the interferon-γ (IFN-γ) promoter through cooperative activation of the nuclear factor kappa B and p38 mitogen-activated protein kinase pathways. Tetracycline-regulated transgenic mice expressing constitutively active Rac2 in T cells exhibited enhanced IFN-γ production. Dominant-negative Rac inhibited IFN-γ production in murine T cells. Moreover, T cells from Rac2−/−mice showed decreased IFN-γ production under TH1 conditions in vitro. Thus, Rac2 activates TH1-specific signaling and IFN-γ gene expression.Keywords
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