The Influence of Ribonucleoside Triphosphates, and Other Factors, on the Formation of Very‐Salt‐Stable RNA‐Polymerase · su+III‐tRNA(tRNATyr)‐Promoter Complexes

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Abstract
The formation of a stable [Escherichia coli] RNA-polymerase [EC 2.7.7.6] .cntdot. su+III-tRNA-promoter complex required sigma factor and the incorporation of ribonucleoside triphosphates which match the 5'' sequence of the su+III tRNA transcript. This complex, stable to at least 2 M KCl, can be retained on a Millipore filter. Its formation closely parallels the extent of transcription obtained from the su+III tRNA promoter in response both to increasing ionic strength and to temperature during incubation of RNA polymerase with the [phage .phi.80] DNA. The RNA-polymerase .cntdot. DNA complex retained during this assay therefore appears to relate directly to that formed during promoter-directed transcription. The formation of RNA-polymerase .cntdot. su+III-tRNA -promoter complexes is sensitive to the presence of ppGpp.