Temperature and salt dependence of the gel migration anomaly of curved DNA fragments
- 12 January 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (1) , 247-265
- https://doi.org/10.1093/nar/15.1.247
Abstract
A series of oligonucleotides of different sequences have been cloned to study DNA curvature. Several DNA fragments containing these oligonucleotides in various numbers of repeats were analyzed in 10% polyacrylamide gels. A strong gel migration anomaly was found for dA 4 sequences; a comparably very small but clearly detectable anomaly was observed for dA 3 (both in a repeat length of 10 base-pairs). The temperature and salt (NaCl, MgCl 2 ) dependence of the gel migration anomaly of these DNA fragments was measured. While a similar behaviour of all sequences is observed for the addition of NaCl, the temperature and MgCl 2 dependence of the anomaly varies with the oligonucleotide sequence. These data are interpreted in terms of local DNA structure changes induced by changes in the temperature and the MgCl 2 concentration which affect the planarity of the curved DNA fragments.Keywords
This publication has 21 references indexed in Scilit:
- A highly bent fragment of Crithidia fasciculata kinetoplast DNA.Journal of Biological Chemistry, 1986
- Visualization of the bent helix in kinetoplast DNA by electron microscopyCell, 1986
- DNA structure: Bent molecules—how and why?Nature, 1986
- Curved DNA: design, synthesis, and circularization.Proceedings of the National Academy of Sciences, 1986
- Sequence dependence of the curvature of DNA: a test of the phasing hypothesisBiochemistry, 1985
- On the sequence determinants and flexibility of the kinetoplast DNA fragment with abnormal gel electrophoretic mobilitiesJournal of Molecular Biology, 1985
- Curved DNCritical Reviews in Biochemistry, 1985
- DNA bending induced by cruciform formationNature, 1985
- Filter disc supported oligonucleotide synthesis by the phosphite methodNucleic Acids Research, 1984
- Anomalous electrophoresis of deoxyribonucleic acid restriction fragments on polyacrylamide gelsBiochemistry, 1983