The Temperature Control System of the Cobas®-Bio Centrifugal Analyzer

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Abstract
The temperature control system of the Cobas-Bio centrifugal analyzer (Roche Analytical Instruments, Montclair, NJ) was evaluated using a calibrated thermistor probe and a solution of cresol red in tris buffer as an “optical thermometer” to measure the temperature of the cuvette contents during operation. The instrument accurately achieves a set temperature of 30°C or 37°C, within thermistor readability of 0.13°C, and maintains that temperature within ±0.05 to 0.08°C for at least 18 minutes. There is negligible temperature difference between cuvettes in a rotor during operation. After pipetting samples and reagents into the rotor, 1.5 minutes at 30°C and 2 minutes at 37°C are required to bring the cuvette contents within 0.1 °C of the set temperature. If a second reagent representing 10% of the total volume is added to cuvettes that have been preincubated at the set temperature, up to 1.5 minutes at 30°C and 2 minutes at 37°C are required to reach temperature equilibrium.

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