Targeting of an inducible toxic phenotype in animal cells.
- 1 October 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (20) , 7572-7576
- https://doi.org/10.1073/pnas.85.20.7572
Abstract
We have developed a toxic, or suicide, vector whose action is based on the targeted expression of the herpes simplex virus 1 thymidine kinase gene product in cultured cells or transgenic animals. This protein is able to convert nucleoside analogs such as acyclovir and 1-(2-deoxy-2-fluoro-.beta.-D-arabinofuranosyl)-5-iodouracil (FIAU) to toxic intermediates. The activation of these compounds disrupts cellular DNA replication, leading to rapid cell death. Neither acyclovir, FIAU, nor the herpes thymidine kinase alone is harmful to cells. This approach is simple and should have widespread applicability in studying lineage formation in cultured cells and transgenic animals.Keywords
This publication has 28 references indexed in Scilit:
- Selective extraction of polyoma DNA from infected mouse cell culturesPublished by Elsevier ,2004
- Transgenic Mice: Applications to the Study of the Nervous SystemAnnual Review of Neuroscience, 1988
- 1-(2-Deoxy-2-fluoro-.beta.-D-arabinofuranosyl)-5-ethyluracil. A highly selective antiherpes simplex agentJournal of Medicinal Chemistry, 1987
- Synergism between immunoglobulin enhancers and promotersNature, 1986
- Introduction of a μ immunoglobulin gene into the mouse germ line: Specific expression in lymphoid cells and synthesis of functional antibodyCell, 1984
- A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genesCell, 1983
- A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain geneCell, 1983
- Immunoglobulin gene transcription is activated by downstream sequence elementsCell, 1983
- SV40-transformed simian cells support the replication of early SV40 mutantsCell, 1981
- Transfer of purified herpes virus thymidine kinase gene to cultured mouse cellsCell, 1977