The short-circuited everted sac of rat colon mucosa
- 1 October 1981
- journal article
- research article
- Published by Springer Nature in Zeitschrift für Die Gesamte Experimentelle Medizin
- Vol. 178 (3) , 247-256
- https://doi.org/10.1007/bf01851014
Abstract
A short-circuited preparation of everted rat colon sacs is described. The serosal current electrode is a AgAgCl wire. A cylindrical agar bridge or AgAgCl electrode may be employed on the mucosal side. Effects of Ag+ ions liberated from the electrodes on ion transport could not be demonstrated. Fluid and sodium are absorbed and bicarbonate secreted. Potassium and chloride movements are not significantly different from zero. The preparation remains stable for at least 2 h. Sodium absorption is diminished by 50% and bicarbonate secretion abolished in the absence of glucose. In principle, similar ion transport properties were found as in Ussing-chamber preparations. The advantage of the everted sac is the capability of measuring net transport of fluid and electrolytes simultaneously and directly because of the large surface/inner volume ratio of the sac.Keywords
This publication has 19 references indexed in Scilit:
- Effect of silver chloride on the short-circuit current across the isolated toad skinLife Sciences, 1977
- Electrolyte transport across isolated large intestinal mucosaAmerican Journal of Physiology-Legacy Content, 1973
- Effects of sugar and amino acid transport on transepithelial fluxes of sodium and chloride of short circuited rat jejunumThe Journal of Physiology, 1972
- Optimum electrolytic chloriding of silver electrodesMedical & Biological Engineering & Computing, 1969
- Electrical potential and short circuit current of an in vitro preparation of rat colon mucosaThe Journal of Physiology, 1968
- Transport of sodium and secretion of potassium and bicarbonate by the colon of normal and sodium‐depleted ratsThe Journal of Physiology, 1967
- Zur resorptionshemmenden Wirkung von Gallens urenNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1966
- Short‐circuit current and solute transfer by rat jejunum.The Journal of Physiology, 1965
- FLUID AND SOLUTE TRANSPORT ACROSS RAT COLONIC MUCOSAQuarterly Journal of Experimental Physiology and Cognate Medical Sciences, 1965
- Measurement of short-circuit current and ion transport across the ileumAmerican Journal of Physiology-Legacy Content, 1964