Measurement of SRS-A activities in plasma of asthmatic patients.

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Abstract
Ethanol extracts of arterial blood from 20 of 29 asthmatic patients during attacks produced sustained contractions of the guinea pig ileum. These contractions were inhibited by FPL 55712, a specific antagonist of slow reacting substance of anaphylaxis (SRS-A [slow-reacting substance of anaphylaxis]). SRS-A activity was significantly higher during attack than during remission. Reverse-phase high pressure liquid chromatography (HPLC) was performed on 2 extracts which showed high SRS-A activity with bioassay. One extract showed double peaks, which had retention times identical to those of leukotriene (LT) C4 and LTD4. The other extract showed a single peak, which had a retention time identical to that of synthetic LTC4.