PROPERTIES OF MONOCLONAL-ANTIBODIES TO HUMAN-IMMUNOGLOBULIN KAPPA AND LAMBDA CHAINS
- 1 January 1981
- journal article
- research article
- Vol. 42 (4) , 649-659
Abstract
Hybridomas were produced from mice immunized with human IgG. Culture supernates were assayed for the presence of antibody-producing cells by passive hemagglutinin. Hybridomas producing antibodies to human kappa (.kappa.) and lambda (.lambda.) L chains were cloned and grown as ascitic tumors in BALB/c mice. The antigen-binding characteristics of the monoclonal antibodies, contained in the ascitic fluid, were assessed by hemagglutination inhibition, enzyme-linked immunosorbent assay and radioimmunoassay systems and by the binding of radiolabeled antigen in analytical flat-bed isoelectric focusing gels. One monoclonal anti-.kappa. reacted better with free than with combined .kappa. chains; for another the reverse was true. Antibody fractions appeared by DEAE chromatography of ascitic fluids were coupled to ox red cells with CrCl5 and compared with polyclonal antibodies for the detection of cell-surface Ig.This publication has 12 references indexed in Scilit:
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