Demonstration of rRNA Synthesis in Pre‐Gastrular Embryos of Xenopus Laevis
Open Access
- 31 December 1980
- journal article
- research article
- Published by Wiley in Development, Growth & Differentiation
- Vol. 23 (6) , 579-587
- https://doi.org/10.1111/j.1440-169x.1981.00579.x
Abstract
Isolated cells from Xenopus laevis embryos at various developmental stages were incubated with (methyl‐3H) methionine to label newly synthesized RNAs. Methylation of RNAs was studied by analyzing nuclease‐digests of high‐molecular‐weight RNAs by DEAE‐Sephadex column chromatography. Labeled rRNA, mRNA and 4s RNA were distinguished by their characteristic patterns of 2′‐0‐methylation and base‐methylation. It was concluded that rRNA synthesis starts during the mid‐ to late‐blastula stage. This is about 4 hr, or at least 3 cell cycles earlier than the initiation of gastrulation, which was previously considered to be the stage when rRNA synthesis begins.This publication has 32 references indexed in Scilit:
- Methylation map of Xenopus laevis ribosomal RNANature, 1980
- Ribosomal RNA synthesis in pre- and post-gastrula-stage sea urchin embryosDevelopmental Biology, 1979
- Partial mapping of methylated sequences in Xenopus laevis ribosomal RNA by preparative hybridization to cloned fragments of ribosomal DNANucleic Acids Research, 1979
- Fine structure of ribosomal RNA. II. Distribution of methylated sequences within Xenopus laevis rRNA*Nucleic Acids Research, 1979
- Poly (A)-containing RNA Synthesis in Xenopus laevis EmbryosCell Structure and Function, 1978
- Methylated blocked 5′ terminal sequences of sea urchin embryo messenger RNA classes containing and lacking poly(A)Cell, 1976
- Methylation of Ribosomal-Precursor RNA, Synthesized in vitro, by Isolated Rat-Liver NucleoliEuropean Journal of Biochemistry, 1975
- RNA synthesis in nuclei isolated from early embryos of Xenopus laevisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Methylation and processing of ribosomal RNA in HeLa cellsJournal of Molecular Biology, 1966
- Synthesis and accumulation of DNA-like RNA during embryogenesis of Xenopus laevisJournal of Molecular Biology, 1966