Regulation of nitrogen fixation (nif) genes of Azospirillum brasilense by nifA and ntr (gln) type gene products
Open Access
- 1 June 1984
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 23 (1) , 95-101
- https://doi.org/10.1016/0378-1097(84)90080-6
Abstract
Eight Nif− mutants of Azospirillum brasilense were obtained by N-nitrosoguanidine mutagenesis and isolated by growth on glutamate medium. Three of these mutants had no nitrogenase activity, possessed no nitrogenase structural proteins and were complemented by Klebsiella pneumoniae nifA. Evidence will be presented that one of these mutants is defective in a nifA type regulatory gene but the other two were also complemented by K. pneumoniae ntrC and may be ntrC−-type mutants. A fourth mutant was defective in the MoFe component protein of nitrogenase.Keywords
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